CJ236 Electrocompetent E. Coli Cells Size:10 x 50 μL Tetracyclines are antibiotics made up

Tetracyclines are antibiotics made up of a four-ring system

02 μg/μL concentration in TE buffer

Prepare solutions just before use

5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid potassium salt

CJ236 Electrocompetent E. Coli Cells Size:10 x 50 μL Tetracyclines are antibiotics made upGoldBio CJ236 Electrocompetent E. coli cells are ideally suited for use in the Kunkel method of mutagenesis. CJ236 cells have part of their Thymine replaced with deoxyuracil in the DNA due to dut and ung mutations which prevent the cells from removing uracil from DNA caused by misincorporation or dCTP deamination. CJ236 contains the F gene which confers Chloramphenicol resistance. Reagents Needed for One Reaction CJ236 electrocompetent cells: 25 L DNA