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For scRNA-seq library preparation, cells were loaded onto the 10 Genomics Chromium instrument and 10 Genomics Chromium Single-Cell 3kit V3 reagents were used according to the manufacturers protocols

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The tour begins at Qorikancha, originally known as the Temple of the Sun, considered the most sacred sanctuary of the Inca Empire where the walls were literally covered in sheets of pure gold, later converted into the Convent of Santo Domingo, creating a fascinating architectural overlay symbolizing the encounter of two civilizations