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The cell lysate was centrifuged at 500 g for 3 times, each lasting 10 min, and then the supernatant was centrifuged for 30 min at 10,000 g

Copper is an essential trace mineral involved in numerous enzymatic reactions, including those governing: collagen crosslinking antioxidant defense connective tissue formation mitochondrial respiration When bound to copper, GHK acts as a carrier molecule that delivers bioavailable copper to cells while simultaneously triggering regenerative signaling pathways

IGF-1R, insulin-like growth factor 1 receptor

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