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Effective long-term weight loss with GLP1 therapy requires ongoing, potentially lifelong use, as discontinuation may result in weight regain
Cells were separated from muscle tissue debris by differential centrifugation and filtration (nylon cell strainers) prior to a final centrifugation step at 1500 x g for 3 min, then suspended in DMEM containing 10% normal horse serum (HS), 1% antibiotic-antimycotic mixture, and 0.5% gentamicin (DMEM-10% HS), and plated on poly-L-lysine and fibronectin-coated plates
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Li YH, He Q, Yu JZ, Liu CY, Feng L, et al
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