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The optical density of each well was determined with the GloMax-Multi Detection System (Promega Corp., Madison, WI Part #TM297) and a regression model was applied to calculate total (i.e., acyl + des-acyl) ghrelin concentrations

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Low-energy shock wave plus intravesical instillation of botulinum toxin A for interstitial cystitis/bladder pain syndrome: pathophysiology and preliminary result of a novel minimally invasive treatment