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Molecular biology methods can be employed to subclone the gene encoding the "bait" protein into an appropriate vector with a fusion tag .Recombinant clones can be overexpressed and easily purified, resulting in an abundance of bait protein for use in pull-down assays

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The effect of one-carbon components supplementation during early gestation on fetal jejunum morphology was recently evaluated ( It is evident that the manipulation of the one-carbon metabolism and the methylation processes through the diet can influence long-term metabolic and performance outcomes

doi: 10.3389/fmicb.2022.957148 Received 30 May 2022 Accepted 26 October 2022 Published 23 November 2022 Volume 13 - 2022 Edited by Vittorio Capozzi, Institute of Sciences of Food Production (CNR), Italy Reviewed by Michele Christine Loewen, National Research Council, Canada