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Metabolism (1995) 44:4751.10.1016/0026-0495(95)90288-0 54 DAlessioDA

The matrix, X, of normalized gene abundances with samples on the rows and the biomarker genes on the columns was provided to the opls function of the ropls package with parameters predI = 2 (derive 2 PCA components) and permI = 100 (perform 100 random permutations of the class labels to evaluate predictive performance) to predict the biological condition (Y)