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For quantification, samples are imaged with an LSM880 laser scanning confocal microscope (Zeiss) or an Operetta high-content imager (Perkin Elmer)

Adding the ~16.2 g absorbed per week from the average of 127 g/day of supplemental B12 for those in the high-risk Brasky group, gives a total of roughly 35.3 g absorbed per week

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