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Hepatic nuclear factor-3 (HNF-3 or Foxa2) regulates glucagon gene transcription by binding to the G1 and G2 promoter elements

monoclonal mouse GLP-1 antibody [8G9] (Abcam, Cambridge, UK MA) diluted 1:3000, pH 9, polyclonal Rabbit 5HT (serotonin) antibody (Immunostar WI, USA) diluted 1/10,000, monoclonal mouse/human SST ghrelin antibody (R&D Systems #MAB8200) or anti-SST antibody (Dako, #A0566) both used at a dilution 1:500

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For the nuclear magnetic resonance (NMR) measurements (Bruker AVANCE NEO 600), we dissolved the fully dried OKGM powder in D 2 O until complete dissolution and then transferred it to an NMR tube using a disposable pipette