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FACS analysis confirmed that at passage 10, 54.7% of EpCAM+ HBs co-expressed the proliferative marker Ki67, and immunostaining showed sustained co-expression of AFP, HNF4, and Ki67

Fluids 37 , 017116 (2025)

Following two consecutive 5-min washing steps in PBS, sections were blocked with 10% (vol./vol.) normal goat serum constituted in low protein blocking solution (eBioscience, San Diego, CA, USA) for 1 h at 37 C, followed by incubation with rabbit anti-GPX1 (1:120, diluted in antibody diluent from Cell Signaling Technology, Danvers, MA, USA) for 1618 h at 4 C

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