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Animal models To evaluate tumor formation ability, limiting dilution assay was conducted by injecting 2 10 3 , 2 10 4 and 2 10 5 MHCC-97L cells subcutaneously into the flanks of male BALB/cAnN-nu nude mice

Most studies rely on single-parameter assessments, and a comprehensive model linking SIRT1 activation efficiency with phenotypic improvement is lacking