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Independent replication outside the Sikiric group exists but is limited

176 In addition to non-selective autophagy induced under starvation conditions, researchers have found that cells can also target the degradation of damaged or redundant organelles, protein aggregates, and invading pathogens for selective autophagy

Huang Y, Zhu L, Li H, Ye J, Lin N, Chen M, Pan D, Chen Z

Assuming you followed the above advice and used an appropriate binding buffer and your protein isnt huge, then a likely reason for this is that the expression yield is much lower than you expected