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Researchers have also observed the increased expression of pro-inflammatory cytokines such as IL-6 and IL-8 in the tumor microenvironment [50]

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Immunofluorescence (IF) for PML bodies 21NT cells (treated with 1ug/ul epitalon and untreated) were plated onto glass microscope slides (Thermo Scientific), then fixed in 2% formaldehyde (Fisher Scientific) for 15 min and cold methanol for 10 min