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For differential expression analysis using the R package limma (27) (see below, section Comparison of group means), 2 parameters estimated by RUVs were included as covariates in the linear model, while the normalized logCPM data without RUVs correction was used as expression matrix input, as is recommended by the authors of the RUVSeq package for this type of analysis

T helper type 1 (Th1) cells derived from initial CD4 + T cell differentiation produce cytokines such as IFN-gamma (IFN-), enhancing immune surveillance by CD8 + T cells [48]

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