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Wear appropriate personal protective equipment (PPE), including a laboratory coat, gloves, and eye protection, with additional controls used where required by institutional safety policies

Samples were grounded in solution comprising of thiobarbituric acid (TBA 0.25%) prepared in trichloroacetic acid (TCA 10%) followed by heating them at 95 C then cooled on ice and centrifuged for 10 min at 10,000 g

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