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Canada Step 4: Replace stress triggers chewing gum hydration breathing exercises quick walks caffeine reduction if anxiety increases Step 5: Track progress count sessions/day track cravings note patterns (stress, boredom, social triggers) Conclusion There is no exact safe number of vape hits per day because vaping exposure differs by device, nicotine strength, puff duration, and behavior patterns

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Most of the methylation loci correlated with RNA expression were located in the gene body and 5-UTR in both LUAD (Supplementary Figure S3a,b) and LUSC (Supplementary Figure S3c,d)