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The ban also means that the production of click dual cigarettes such as Sterling Dual that change from normal to menthol, has been halted

The nucleic acid remaining in the supernatant was precipitated by adding 4 volumes of 95% ethanol, 1/10 volume of sodium acetate, flash frozen in liquid nitrogen, thawed on ice, and pelleted by centrifugation at 30,000xg for 30 min at 4C

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Smoking- and E-liquid-induced BCEVs are genotoxic and promote oxidative stress Smoking is a major risk factor for BC development, and work from our laboratory has previously demonstrated the ability of BCEVs to contribute to genotoxic stress in recipient urothelial cells, thereby contributing to their malignant transformation 40