Mehta Department of Public Health, Arba Minch University, Arba Minch, Ethiopia Tesfahun Mekene Meto School of Nursing and Midwifery, Deakin University, Melbourne, Victoria, Australia Birye Dessalegn Mekonnen Department of Medical Laboratory Sciences, Adigrat University, Adigrat, Ethiopia Hadush Negash Meles Department of Internal Medicine, University of Gondar, Gondar, Ethiopia Endalkachew Belayneh Melese Johns Hopkins University, Baltimore, MD, USA Endalkachew Belayneh Melese Department of Medical Oncology and Hematology, Kantonsspital St
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Abstract Tobacco Etch virus (TEV) protease is one of the most common tools for removing fusion tags, but no study has shown that TEV can be expressed at high levels in the GRAS host strain Bacillus subtilis and purified for further application