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Supernatant proteins were precipitated using trichloroacetic acid, subjected to SDS-PAGE and separated proteins blotted onto nitrocellulose membrane as described previously 44

(2008: 1691) cautioned against using concepts of self-medication to rationalize allowing ongoing tobacco use and limited smoking cessation efforts in many mental health treatment settings. As the authors further illustrated, smoking in psychiatric facilities may be not only passively accepted but also implicitly encouraged: the habit provides opportunities for social contact among inpatients without the clinicians intruding on them