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It continued to fuel the colonial economy, contributed to the unrest which resulted in the American War of Independence (1775-1783 CE), increased tensions in the country leading up to the American Civil War (1861-1865 CE), and was the cause of the Tobacco Wars of the early 20th century CE

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Cobas EGFR Mutation Test v2 identifies 42 mutations in exons 18, 19, 20, and 21 of the epidermal growth factor receptor (EGFR) gene

Harmful effects of tobacco: The inhalation of smoke causes the absorption of chemicals by the tissues and leads to the following harmful effects: Tobacco smoke contains benzopyrene and polycyclic hydrocarbons, which are carcinogenic, causing lung cancer