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For TUNEL IF detection, slides were incubated with lectin PNA, Alexa Fluor 594 conjugate (Invitrogen, L32459) at room temperature for 1 h in the dark, and then treated with One Step TUNEL Apoptosis Assay Kit (Beyotime, C1088)
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But the Bitterroot ecosystem has always been a place biologists and wildlife managers hypothesized bears would eventually repopulate on their own
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