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One thousand nanograms RNA from each sample was used as a template for preparing Illumina compatible libraries using the TruSeq RNA Library Prep Kit v2 (Illumina)

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The symptoms are EXACTLY as young Chance and my son reported and thankfully in the case of my son, once the lung was re-inflated he needed no further treatment and was certainly, and unlike Chance, not told to stop long distance runningthough in my sons case he tends to run away quite briskly when its his round at the bar And most importantly, my son has NEVER smoked nor vaped in his life