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The American Society for Reproductive Medicine notes that the clinical value of DNA fragmentation testing is not recommended to evaluate male infertility

The cultures were then grown to the stationary phase in a shaking water bath for 18 h at 30C, after which time the yeast cells were collected, washed with phosphate-buffered saline (PBS), counted by means of a haemocytometer, and adjusted to 10 6 /ml prior to use

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