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Similar to a dark Burley, this leaf contained roughly three to nine times the nicotine content of most modern tobaccos (depending on the varietal), and was burned for recreation as often as it was for religious ceremony

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Then, the components in the distillation flask were washed out with 0.1% BHT methanol solution, transferred to a volumetric flask (5 mL), and fixed with 0.1% BHT methanol solution