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When NO donor was added to tobacco induced cells, development of partial cell deaths was observed which confirmed the role of NO as the cell death mediator Biomarkers Major nicotine metabolite Cotinine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) Essentiality and deficiency (specific to metals) : N/A References Engstrom P.F., Clapper, M.L and Schnol, R.A