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DNase was diluted 2-fold with HBSS, cells were centrifuged 225 g , 5 min, 4 C, and seeded onto bovine collagen-coated 6.5 mm transwell inserts (2 10 4 cells/insert) in ALI Expansion medium (50% BEBM, 50% DMEM-C, 0.5 mg/mL BSA, 80 M ethanolamine, 10 ng/mL hEGF, 0.4 M MgSO 4 , 0.3 M MgCl 2 , 1 M CaCl 2 , 30 ng/mL retinoic acid, 0.8 insulin*, 0.5 transferrin*, 1 hydrocortisone*, 1 epinephrine*, 1 bovine pituitary extract*, 1 gentamicin/amphotericin*, *relative to BEGM Bullet Kit aliquot) with RI (day 5)

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Within two weeks of commencing employment the applicant developed a reaction to the cigarette smoke and got sick