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Histology and immunofluorescence Organoids were washed to remove basement membrane extract, fixed in 4% paraformaldehyde, embedded in Histogel, paraffin-processed, sectioned (4 m) and stained according to standard hematoxylin and eosin (H&E) protocols

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Nevertheless, pronounced year-to-year fluctuations within sites suggest that the underlying dynamics are complex and potentially nonlinear

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