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iNOS/eNOS Western-Blot CSS-treated RBL-2H3 and A549 cells were rinsed with PBS and isolated by scraping in ice cold radio-immunoprecipitation (RIPA) buffer (1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS in PBS) with freshly added aprotinin (30 l/mL RIPA)

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Although we replicated the findings in former smokers from the MCPS cohort and found overall consistent associations of CHRNB3 rare variants with cig per day across different ancestries, we lacked an independent Mexican ancestry cohort for direct replication of the p.Glu284Gly variant