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The thwarted love interest in the story read it and she was quite upset by it at first, but their relationship and their friendship recovered and became a lot stronger and healthier because of it

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The cultures were then grown to the stationary phase in a shaking water bath for 18 h at 30C, after which time the yeast cells were collected, washed with phosphate-buffered saline (PBS), counted by means of a haemocytometer, and adjusted to 10 6 /ml prior to use