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For tobacco-related samples however, with a large nicotine peak, 2MN eluted on the tail of nicotine, which will always create a problem for low-resolution-MS-based confirmation of the presence of a low level of 2MN because with m / z = 84 amu as the MS base peak for both nicotine and 2MN, obtaining a mass spectrum for a small candidate 2MN peak using subtraction of the background signal from a large nicotine tail will not result in a properly-sized m / z = 84 amu signal for proper MS pattern concordance

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